Sanger Sequencing and genotyping

Sanger sequencing is a method of DNA sequencing that involves electrophoresis and is based on the random incorporation of chain-terminating dideoxynucleotides by DNA polymerase during in vitro DNA replication. This method sequences a single strand of DNA up to around 800 base pairs in length. All of the DNA in the sample must be the same for this method to work (please no mixed community samples, like 16S from a soil sample).

Genotyping Genotyping is the process of determining the DNA sequence, called a genotype, at positions within the genome of an individual. Sequence variations can be used as markers in linkage and association studies to determine genes relevant to specific traits.

Information around sample requirements and pricing can be found on iLAB or email us if you have questions.